منابع مشابه
Detecting Glycosylations in Complex Samples
Glycoproteins are the highly diverse key element in the process of cell – cell recognition and host – pathogen interaction. It is this diversity that makes it a challenge to identify the glyco-peptides together with their modification from trypsin-digested complex samples in mass spectrometry studies. The biological approach is to isolate the peptides and separate them from their glycosylation ...
متن کاملChemical O‐Glycosylations: An Overview
The development of glycobiology relies on the sources of particular oligosaccharides in their purest forms. As the isolation of the oligosaccharide structures from natural sources is not a reliable option for providing samples with homogeneity, chemical means become pertinent. The growing demand for diverse oligosaccharide structures has prompted the advancement of chemical strategies to stitch...
متن کاملStereoselective glycosylations using oxathiane spiroketal glycosyl donors.
Novel oxathiane spiroketal donors have been synthesised and activated via an umpolung S-arylation strategy using 1,3,5-trimethoxybenzene and 1,3-dimethoxybenzene. The comparative reactivity of the resulting 2,4,6-trimethoxyphenyl (TMP)- and 2,4-dimethoxyphenyl (DMP)-oxathiane spiroketal sulfonium ions is discussed, and their α-stereoselectivity in glycosylation reactions is compared to the anal...
متن کاملDefining oxyanion reactivities in base-promoted glycosylations.
Saccharide oxyanions obtained by base treatment could be employed in glycosylation to give oligosaccharides with high stereo- and regioselectivities.
متن کاملCharacterization of kallikrein-related peptidase 4 glycosylations.
Kallikrein-related peptidase 4 (KLK4) is a glycosylated serine protease that functions in the maturation (hardening) of dental enamel. Pig and mouse KLK4 contain three potential N-glycosylation sites. We isolated KLK4 from developing pig and mouse molars and characterized their N-glycosylations. N-glycans were enzymatically released by digestion with N-glycosidase F and fluorescently labeled wi...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2003
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb1614iti4